Ernesto Zanotto

 

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Ernesto Zanotto
PhD Student

Master degree in  Medicinal Chemistry and Technology, Faculty of  Pharmacy, University of Padova, Italy

Tel: +358335517732, +358335517727 (lab)
Fax: +358335517710
E-mail: ernesto.zanotto (at) uta.fi

Project: Models of mitochondrial disease

Techniques:

  • molecular biology: basic techniques in molecular biology; cloning, sequencing
  • cell-culture techniques: immortalized cells, primary hepatocytes (rat, mouse) 
  • mitochondrial biochemistry
    • Polarography techniques. oxygen consumption in intact isolated mitochondria, drug testing, anoxia and reoxigenation 
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    • Spectrophotometry-Fluorometry: biochemistry of the respiratory chain, mitochondrial membrane fluidity, membrane potential and swelling
  • qPCR
  • DNA-Protein Interaction
    • EMSA
    • in vitro DNaseI footprinting
    • in vivo DMS footprinting
    • Chromatine immunoprecipitation
      •



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